Wilko employee said their final goodbyes with a dance performed to the tune of “So Long, Farewell”.
Hayley Burrows, 37, took part in the video along with seven colleagues after their last customer left the shop.
The video shows the Wilko workers - some in uniform - take their final bows as they bid farewell to the store.
The shop in Minstergate, Thetford, Norfolk had been open since 2008, but its closure comes after the company hired administrators last month, announcing the closure of its 400 remaining locations.