The Bee Gees performed songs from every decade from the 1960s to the '90s. They also sang a tribute song to their late brother Andy Gibb, "(Our Love) Don't Throw It All Away". http://smarturl.it/beegees1nightonlydvd During this song old footage of Andy is shown, including him singing the second verse of the song. The vocals from the original recording also play during that section. Céline Dion guest-starred on the Bee Gees-penned "Immortality". Recorded vocals by Frankie Valli are featured during "Grease", and are also taken from the original song.

The album's title was originally meant to reflect the band's plan that the Las Vegas concert would be their final live performance ever. Barry Gibb's arthritis had worsened to the point where it seemed it would be impossible for him to continue playing, but he wanted to expand the tour and so they played several more shows.